Structure of Transcripts and Proteins Encoded by U79-80 of Human Herpesvirus 6 and Its Subcellular Localization in Infected Cells
Identifieur interne : 003693 ( Main/Exploration ); précédent : 003692; suivant : 003694Structure of Transcripts and Proteins Encoded by U79-80 of Human Herpesvirus 6 and Its Subcellular Localization in Infected Cells
Auteurs : Tomokuni Taniguchi [Japon] ; Takuya Shimamoto [Japon] ; Yuji Isegawa [Japon] ; Kazuhiro Kondo [Japon] ; Koichi Yamanishi [Japon]Source :
- Virology [ 0042-6822 ] ; 2000.
English descriptors
- Teeft :
- Acceptor sites, Amino, Amino acid similarity, Binding protein, Bruyn kops, Cdna, Cdna clones, Cells transfected, Clone, Common peptide, Complex alternative, Cytomegalovirus, Distribution pattern, Early genes, Encoding, Exon, Expression level, Expression vector, Fragment encoding, Fusion protein, Gene, Gene products, Genome, Genomic, Gompels, Hcmv, Herpesvirus, Human cytomegalovirus, Human herpesvirus, Human infection, Indirect immunofluorescence assay, Individual proteins, Inhibitor, Intranuclear, Isegawa, Localization, Lysates, Mocarski, Northern blot analysis, Nucleotide sequence analysis, Open reading frames, Other genes, Parallel lanes, Peptide, Plasmid, Polymerase, Polymerase processivity factor, Primary infection, Primer, Primer pair, Protein, Protein expression, Rabbit polyclonal antibody, Replication, Replication compartments, Replication machinery, Room temperature, Rpmi, Sequence coordinates, Sequencing, Specific proteins, Spector, Subcellular, Subcellular localization, Taniguchi, Total clones, Transcript, Transfected, Transfected cells, Viral, Virol, Virology, Yamamoto, Yamanishi.
Abstract
Abstract: We analyzed the U79-80 gene of human herpesvirus 6 (HHV-6), which is predicted to be a positional homolog of the UL112-113 gene of human cytomegalovirus (HCMV). The U79-80 gene encoded a family of nuclear proteins of 36, 41, 44, and 59 kDa. These proteins had common amino termini and were generated by complex alternative splicing. Transcripts from U79-80 appeared as early as 3 h postinfection and could be detected in the presence of phosphonoformate. U79-80 proteins were seen as early as 8 h postinfection and could be detected in the presence of phosphonoformate but not in the presence of cycloheximide combined with actinomycin D treatment. The U79-80 proteins were localized to the nucleus of infected cells, where they were detected as a speckled or punctuate pattern. Moreover, the U79-80 proteins colocalized with the components of the viral DNA replication machinery and appeared to distribute adjacent to or touching nuclear domain 10, where viral DNA replication occurs. From the sequence analysis of genomic DNA, the predicted amino acid similarity between U79-80 and UL112-113 was lower than between other genes, but the characteristics of the transcripts and proteins encoded by U79-80 were similar to those of UL112-113. These results suggest that the U79-80 proteins have a role in viral DNA replication and are functional homologues of the UL112-113 proteins.
Url:
DOI: 10.1006/viro.2000.0326
Affiliations:
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infection</term><term>Primer</term><term>Primer pair</term><term>Protein</term><term>Protein expression</term><term>Rabbit polyclonal antibody</term><term>Replication</term><term>Replication compartments</term><term>Replication machinery</term><term>Room temperature</term><term>Rpmi</term><term>Sequence coordinates</term><term>Sequencing</term><term>Specific proteins</term><term>Spector</term><term>Subcellular</term><term>Subcellular localization</term><term>Taniguchi</term><term>Total clones</term><term>Transcript</term><term>Transfected</term><term>Transfected cells</term><term>Viral</term><term>Virol</term><term>Virology</term><term>Yamamoto</term><term>Yamanishi</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: We analyzed the U79-80 gene of human herpesvirus 6 (HHV-6), which is predicted to be a positional homolog of the UL112-113 gene of human cytomegalovirus (HCMV). The U79-80 gene encoded a family of nuclear proteins of 36, 41, 44, and 59 kDa. These proteins had common amino termini and were generated by complex alternative splicing. Transcripts from U79-80 appeared as early as 3 h postinfection and could be detected in the presence of phosphonoformate. U79-80 proteins were seen as early as 8 h postinfection and could be detected in the presence of phosphonoformate but not in the presence of cycloheximide combined with actinomycin D treatment. The U79-80 proteins were localized to the nucleus of infected cells, where they were detected as a speckled or punctuate pattern. Moreover, the U79-80 proteins colocalized with the components of the viral DNA replication machinery and appeared to distribute adjacent to or touching nuclear domain 10, where viral DNA replication occurs. From the sequence analysis of genomic DNA, the predicted amino acid similarity between U79-80 and UL112-113 was lower than between other genes, but the characteristics of the transcripts and proteins encoded by U79-80 were similar to those of UL112-113. These results suggest that the U79-80 proteins have a role in viral DNA replication and are functional homologues of the UL112-113 proteins.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Taniguchi, Tomokuni" sort="Taniguchi, Tomokuni" uniqKey="Taniguchi T" first="Tomokuni" last="Taniguchi">Tomokuni Taniguchi</name></noRegion><name sortKey="Isegawa, Yuji" sort="Isegawa, Yuji" uniqKey="Isegawa Y" first="Yuji" last="Isegawa">Yuji Isegawa</name><name sortKey="Kondo, Kazuhiro" sort="Kondo, Kazuhiro" uniqKey="Kondo K" first="Kazuhiro" last="Kondo">Kazuhiro Kondo</name><name sortKey="Shimamoto, Takuya" sort="Shimamoto, Takuya" uniqKey="Shimamoto T" first="Takuya" last="Shimamoto">Takuya Shimamoto</name><name sortKey="Yamanishi, Koichi" sort="Yamanishi, Koichi" uniqKey="Yamanishi K" first="Koichi" last="Yamanishi">Koichi Yamanishi</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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